[Analysis on bioactivity of HIV-1 integrase by ELISA method].

Objective: To develop an ELISA-based method for analyzing biologic activities of HIV-1 integrase and for high throughput screening of integrase inhibitors. Methods: After expression,renaturation and purification of integrase,the bioactivity of integrase and the inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and chemiluminescent substrates. Results: (1)The specific activity of the purified integrase was 54.92 units/mg of protein.(2)IC50 (concentration causing 50% inhibition of integrase) of luffin-a was (0.63±0.026)μmol/L. Conclusion: The non-radioactive assay can be used for analysis of bioactivities and high throughput screening of inhibitors of HIV-1 integrase.