[Effect of Zuoguiyin on expression of ovarian VEGF and SPARC in rats during peri-menopausal period].

Objective:To investigate the effect of one kind of regulating-qi and Nourishing-yin Chinese herbs Zuoguiyin on the expression of rats ovarian vascular endothelial growth factor(VEGF) and secreted protein acidic rich in cysteine(SPARC) during the period of peri-menopause.Method:The animal models of perimenopause rats were established by natural aging.Perimenopause rats were treated by intragastric administration(ig) with low(13.78 g·kg-1),middle(20.67 g·kg-1) and high(31 g·kg-1) dose of Zuoguiyin for 8 weeks.Expression of VEGF and SPARC mRNA were detected by RT-PCR.Immunohistochemistry was used to evaluate expression levels of VEGF protein.Result:Compared with that in the control group,ovarian expression levels of VEGF mRNA and its protein in rats during peri-menopause increased significantly(P0.01).Middle and high dose of Zuoguiyin could both down-regulate the expression of VEGF in ovaries of praesenilis rats,and the difference has statistical significance(P0.01).Expression levels of SPARC mRNA in rat ovaries during peri-menopausal period decreased obviously compared to that in the control group(P0.01).Middle and high dose of Zuoguiyin could greatly promote ovarian SPARC mRNA expression of praesenilis rats(P0.01).Conclusion:The abnormal changes of VEGF and SPARC may play an important role in the aging process of ovary.Zuoguiyin,one kind of regulating-qi and Nourishing-yin Chinese herbs,can improve ovarian vascular formation through down-regulating the expression of VEGF meanwhile up-regulating the expression of SPARC.And it may delay aging by this way.