Three-Dimensional Culture of Human Uterine Smooth Muscle Myocytes on a Resorbable Scaffolding

The objective of this study was to develop a three-dimensional culture system for the study of human myometrial physiology. Primary cell lines were initiated from human myometrium obtained at the time of term cesarean delivery. After several passages, cells were seeded onto a polyglactin-910 (Vicryl) mesh and maintained in culture. After several days in culture, each mesh was transferred to another culture dish and suspended to avoid contact with the plastic of the dish. Time-lapse videomicroscopy was used to observe cell proliferation and three-dimensional (3-D) fill of the pores of the mesh. Membrane potentials of the cells of this 3-D tissue were measured with a conventional microelectrode. Confocal microscopy was used to assess 3-D morphology. In some experiments, cells were seeded onto two layers of mesh and then cultured as described above. In this two-mesh experiment, force was measured by anchoring one mesh and pulling on the other, using a micrometer-driven strain gauge. In the single-mesh experi...