DEVELOPMENT AND VALIDATION OF THE HPLC-PROCEDURE OF METOCLOPRAMIDE DETERMINATION IN BLOOD

The HPLC-procedure of metoclopramide quantitative determination in blood using amphiphylic solvent (acetonitrile) for analyte isolation at рН = 5 with further separation of organic layer under the conditions of aqueous phase saturation by ammonium sulphate has been developed for application in forensic and toxicological analysis. Validation of the developed procedure has been carried and the possibility of application of the method of calibration curve, method of standard and method of additions for metoclopramide quantitative determination in blood by the method of HPLC has been shown.