Investigation of the development ability of tooth germ components from different stages

Objective: To investigate the potential of the differentiation and morphogenesis of tooth germs from separate development stage to provide an effective strategy for tooth regeneration research.Methods: Tooth germs of lower first molars from 14-day embryonic and one-day postnatal Sprague-Dawley rats were investigated the ectopic growth and development in renal capsules.Results: The HE results demonstrated that the tooth germ-related tissues at cap stage that could form regular tooth structures developed much better than those at late bell stage.Conclusion: In conclusion,dissociated tooth germs from early stage could recapitulate the embryonic tooth germ environment to favour normal tooth development,indicating that genetic signaling network from early development of tooth germ is necessary for odontogenesis.dental epithelial and mesenchymal tissues.