中藥藥材六味地黃丸、菊杞地黃丸與養血壯筋丸經鈷－60輻射滅菌後之致突變試驗研究

This paper reported the use of transformed salmonella (TA100 and TA98) in the mutagenesis test for the radiated Chinese medicines. In this experiment the TA100 strain is used to detect the base-pair substitution mutagenesis, and the TA98 strain is used to detect the frame-shift mutagenesis. The Chinese medicals were irradiated for 0kGy, 5kGy, 10kGy, 18kGy, 25kGy and 30kGy. Each medical was then mixed with the transformed salmonella (TA100 and TA98) and transfer into the low glucose media plate. After incubated at 37℃ for 48~72 hours, the Ames standard protocol is used to analysis the mutagenesis ratio. To colony number growth on the plate calculated to make the score of the test In Ames test we could not found any different mutagenesis between the controls and radiated Chinese medical. So it was concluded that the radiation won't induce any mutagenesis to the Chinese Medical.