High performance liquid detection method for content of D-Carnitine in levocarnitine and levocarnitine salt product
2015
The invention discloses a high performance liquid detection method for the contents of D-Carnitine in levocarnitine and a levocarnitine product, and belongs to the field of medicament detection. According to the method, a chiral stationary phase high performance liquid chromatography method is adopted; a chiral stationary phase is formed by bonding cyclodextrin and a derivative of cyclodextrin with a silica gel filler, so that the separation effect of three components such as levocarnitine, D-Carnitine and levocarnitine impurities A is good, and the method is high in repetitiveness and durability; a flowing phase adopts a triethylamine-acetic acid aqueous solution and an acetonitrile system; the flowing phase is basically lossless relative to a chiral chromatographic column; the chromatographic peak retention time is high in repetitiveness, and the peak type is symmetric.
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