In vitro antitubercular activity of extract and constituents from the stem bark of Disthemonanthus benthamianus

Abstract A new C -glycosylflavone, apigenin 7-methyl ether 6- C -[β-xylopyranosyl-(1→3)-β-glucopyranoside] named distemonanthoside was isolated from the stem bark of Distemonanthus benthamianus Baill., Fabaceae, along with six known compounds, sitosterol 3- O -β- d -glucopyranoside, 4-methoxygallic acid, syringic acid, quercetin, 6″- O -acetylvitexin, quercetin 3- O -β- d -glucopyranoside. The structures of those compounds and others were determined through spectral analyses. Compounds distemonanthoside, sitosterol 3- O -β- d -glucopyranoside, 4-methoxygallic acid and quercetin were tested against a clinical isolate strain of Mycobacterium tuberculosis AC 45; they exhibited good to moderate antitubercular activities with MIC values ranged from 31.25 to 125 μg/ml.