Prokaryotic Expression and Purification of Heat Shock Factor HSF1 in Arabidopsis thaliana
2009
[Objective] This study was to express and purify Arabidopsis thaliana heat shock factor HSF1. [Method] Using Escherichia coli M15 harboring HSF1 (pQE32/His6-HSF1,pREP4) as experimental materials,HSF1 was induced to express with isopropy1-β-D-galactoside (IPTG);then the expression product was purified using Ni-NTA-agarose affinity chromatography and analyzed by SDS-PAGE. [Result] HSF1 of Arabidopsis thaliana was successfully expressed and purified. [Conclusion] This study provides materials for understanding the blinding site of HSF1 on Arabidopsis thaliana chromosome,further laying a good foundation for revealing the regulatory mechanism and physiological function of HSF1.
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