A New Method for Quickly Preparing Small RNA Molecular Mass Marker

A self cleaving ribozyme was comprised of a hammerhead ribozyme and its target sequence located the downstream of the hammerhead ribozyme . The self cleaving ribozyme gene was synthesized , amplified and cloned into the Bluescript SK plasmid. The construct harboring 10 copies of the self cleaving ribozyme gene was obtained through successively cloning for four times. The result of polyacrylamid denatured gel electrophoresis showed: the multimeric ribozymes caused self cleaving during the transcription reaction in vitro and formed RNA step ladders from 70 nt to 706 nt, which indicates that the self cleavage ribozyme transcripts can be used as RNA molecular mass markers.