한약재 주정 추출물이 종자발아와 유묘생장에 미치는 영향

BACKGROUND: Herbal extracts have been screened fortheir inhibitory effect of seed germination and rootdevelopment on weeds, but there is a scarcity of reports forcrop growth regulation. The objective of this research wasto develop a growth inhibitor on Brassica campestris, andits effective extraction method from herbal medicineextract. METHODS AND RESULTS: Eighty four herbal medicineextracts were tested for their plant growth inhibitionactivity on B. campestris. The alcohol extracts of Artemisiaannua, Cinnamomum cassia, and Mentha arvensisinhibited over 30% of germination and the extract of A. annua, and C. cassia inhibited over 70% of radicle growthat 0.1 % w/w treatment. The partially purified extracts of A. annua, and C. cassia with dichloromethane and hexaneshowed stronger radicle growth inhibition than the crudeextracts on B. campestris. The diethyl ether extract of A. annua showed a similar 50% radicle growth inhibition (RI50= 45 mg/L) to its partially purified extract withdichloromethane or hexane, but the diethyl ether extract ofC. cassia showed a worse RI50 than the purified extract. CONCLUSION: The alcohol extracts of A. annua, and C. cassia showed potent radicle growth inhibition propertieson B. campestris. Diethyl ether proved to be a good solventfor simple extraction from A. annua.