Anthelmintic and α-amylase Inhibition Effects of Ethanol Extract and Its Different Fractions of Rhaphidophora Glauca (Wall.) Schott Leaves

Objective: To investigate the therapeutic effects of ethanol extract and its fractions of Rhaphidophora glauca leaves in anthelmintic (in vitro) and α-amylase inhibitory activity (in vitro). Methods: Leaves of Rhaphidophora glauca was extracted with pure ethanol and fractioned with chloroform (CHFRG), petroleum ether (PEFRG), n-hexane (NHFRG) and ethyl acetate (EAFRG), which are tested for anthelmintic activity on aquarium worm Tubifex tubifex by using three concentrations viz., 5, 10 and 20 mg/ml of each. α-amylase inhibitory activity estimated by modified starch iodine protocol. Result: Among the EERG and various fractions, PEFRG exhibited strong anthelmintic activity in vitro. Where it paralyzed (4.54±0.39 min) and produced death (8.37±0.86 min) of the Tubifex tubifex at 20 mg/ml dose near the value of the standard, Levamisole (3.3±0.38 min and 6.5±0.76 min) at 1 mg/ml. EERG and its all fractions showed good α-amylase inhibitory activity, but PEFRG [IC50=1.757±0.025mg/ml] showed highest activity among them as compared to Acarbose. Conclusion: These findings suggest that the plant may be a potential source for the development of new anthelmintic and α-amylase inhibitory agent.