Comparative study of small linear and branched α-glucans using size exclusion chromatography and static and dynamic light scattering

A series of synthesized small linear and branched R-glucans has been studied by dynamic light scattering and combined size exclusion chromatography, refractive index measurement and static light scattering. The R-glucan molecules studied were maltose, maltotriose, maltopentaose, maltohexaose, maltoheptaose, panose, 6′-R-maltosyl-maltotriose, methyl 6′-R-maltosyl-maltotrioside, 6′′-R-maltosyl-maltotetraose, 6′′′-R-maltotriosyl-maltohexaose, and 6,6′′′′-bis(R-maltosyl)-maltohexaose. The R-glucan oligosaccharides appeared to be very flexible molecules having a variety of conformations and self-associating into noncovalent dimers and trimers (referring to the single molecule). The size distributions were narrow (compared to pullulan) indicating that the R-glucan oligosaccharides are relatively compact molecules. The branched oligomers that include one or more flexible R-(1 f 6) linkages exhibit size distributions corresponding to more compact conformations than their linear counterparts. This observation may be explained by intermolecular interactions or water bridges facilitated by the additional flexibility of these molecules. For the branched maltohexaose, a significant noncovalent trimer formation was observed, whereas in all other cases, noncovalent dimers were formed. Model calculations suggest that both the linear and branched oligomers containing 5-10 R-glucose units exist predominantly in a partial or full single turn helix in agreement with the glycosidic linkage preferences derived for these molecules.