IMMUNOFLUORESCENT METHODS IN THE DIAGNOSIS OF INFECTIOUS DISEASES

Abstract : The fluorescent antibody methods are utilized in microbiological diagnosis to determine either (a) the presence of unknown antigens (usually microorganisms) in tissue sections or smears, or (b) the presence of an unknown antibody in the serum of the patient. There are four methods by which one or both objectives can be achieved: (1) Direct; (2) Indirect; (3) Inhibition, and (4) Complement staining. The direct and indirect methods have been used to a greater extent than the last two procedures and are discussed with more detail. Since the principles of IF depend on the method used, they are also described in the report.