Meloidogyne incognita: Molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase

Abstract Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita . Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like ( Mi-asp1 ) transcript was isolated from second-stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank™ ( DQ360827 ) sequences showed 59–71% identity with nematode-specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita . Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control.