Phorbol esters stimulate a phospholipase D-catalysed reaction with both ester- and ether-linked phospholipids in HeLa cells

Abstract The phorbol ester, 12-tetradecanoylphorbol-13-acetate (TPA), stimulated the accumulation of labelled phosphatidylethanol (PEth) in HeLa cells prelabelled with [ 3 H]hexadecanol and incubated in the presence of ethanol. PEth formation involves a phospholipase D-catalysed phosphatidyl transferase reaction. [ 3 H]Hexadecanol labelled both ester- and ether-derivatives of HeLa cell phospholipids and TPA stimulated the conversion of both classes of lipid to PEth. Phorbol ester; Phospholipase D; Ether phospholipid