Determination of α-Lactalbumin in Infant Formula Milk Powder by High Performance Liquid Chromatography

A high performance liquid chromatographic method was established to determine native and denatured α-lactalbumin in infant formula milk powder.Guanidine hydrochloride buffer was used as both sample extraction solution and HPLC mobile phase.Beta-mercaptoethanol was used as reductant for sample pretreatment.The chromatographic separation was performed on a TSK-GEL G3000SWXL column and the pooled eluate was detected at 280 nm.The quantification was carried out using breast milk as internal standard.Good separation and accurate determination of α-lactalbumin in infant formula milk powder was completed using the method in around 20 min.The method was fast,accurate,and suitable for the determination of α-lactalbumin in infant formula milk powder.