Purification of Flavonoids from RADIX RHIZOMA GLYCYRRHIZAE by Macroporous Resins

[Objective] To optimize the purification conditions of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE by macroporous resins.[Method] 5 kinds of macroporous resins including ADS-7,HPD-300,D-101,HPD-910 and AB-8 were adopted to adsorb flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.Besides,effects of factors affecting the adsorption properties such as sample concentration,ethanol concentration and ethanol volume were also investigated.[Result] ADS-7 was provided with higher absorption rate and the elution rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE,the optimum effect of which were obtained with sample concentration at 1.2 mg/ml using 5 BV of 70% ethanol as eluent.After purification,the content and refined rate of flavonoids in powder were 47.1% and 211.2%,respectively.[Conclusion] ADS-7 resin had strong adsorption and purification effects,which would provide reference basis for purifying flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.