Genome-wide association study for nine plant architecture traits in Sorghum
2016
Sorghum [Sorghum bicolor (L) Moench], an important grain and
forage crop, is receiving significant attention as a lignocellulosic
feedstock because of its water-use efficiency and high biomass
yield potential. Because of the advancement of genotyping
and sequencing technologies, genome-wide association study
(GWAS) has become a routinely used method to investigate the
genetic mechanisms underlying natural phenotypic variation. In
this study, we performed a GWAS for nine grain and biomassrelated
plant architecture traits to determine their overall genetic
architecture and the specific association of allelic variants in
gibberellin (GA) biosynthesis and signaling genes with these
phenotypes. A total of 101 single-nucleotide polymorphism (SNP)
representative regions were associated with at least one of the
nine traits, and two of the significant markers correspond to GA
candidate genes, GA2ox5 (Sb09 g028360) and KS3 (Sb06
g028210), affecting plant height and seed number, respectively.
The resolution of a previously reported quantitative trait loci (QTL)
for leaf angle on chromosome 7 was increased to a 1.67 Mb
region containing seven candidate genes with good prospects
for further investigation. This study provides new knowledge of
the association of GA genes with plant architecture traits and the
genomic regions controlling variation in leaf angle, stem circumference,
internode number, tiller number, seed number, panicle
exsertion, and panicle length. The GA gene affecting seed number
variation (KS3, Sb06 g028210) and the genomic region on
chromosome 7 associated with variation in leaf angle are also
important outcomes of this study and represent the foundation
of future validation studies needed to apply this knowledge in
breeding programs.
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