PCR testing and identification optimizing process for carrot black rot

This invention is PCR method used to detect and determine carrot black rot germ. It is propitious to detect carrot black rot and earlier diagnoses, and it can exactly determine the Alternaria radicina of the disease. It belongs to crop disease protection field. This method detects the germ according to the special primer brought by the germ, the amplifying product is 343bp. At the same time, the amplification program of the PCR is optimized by this method, 94 degree centigrade pre-changing 4min is added, and the sensibility is improved. It is the supplement and improvement of the method of detecting carrot black rot germ special prime.