Citokininek hatásvizsgálata alma in vitro hajtás regenerációjában = Role of cytokinins in shoot regeneration of apple in vitro

Hat alma fajtanal (Royal Gala, M.26, Idared, Freedom, McIntosh, Husveti rozmaring) fajtankent optimalizaltuk az előkezelő taptalaj citokinin tartalmat (citokinin tipusa es koncentracioja), tisztaztuk hatasukat az adott genotipus morfogenetikus kepessegere. Kimutattuk a kulonboző tipusu citokininek kulonboző koncentracioja okozta szoveti valtozasokat az in vitro hajtasok leveleiben. Megallapitottuk, hogy a regeneraciora hasznalt levelek szoveti szerkezete es a regeneracios valaszuk jo korrelaciot mutat. Fajtankent optimalizaltuk a regeneracios taptalaj citokinin tartalmat. Kimutattuk a kulonboző citokininek es az alma fajtak kozotti kulonbsegeket a hajtasok differencialodasanak időbeli alakulasaban. Elvegeztuk 65 almafajta molekularis analiziset es elkuloniteset mikroszatellit primerekkel (SSR markerek). Elkeszitettuk a 6 SSR lokusz alapjan a fajtak dendrogramjat is, amely az ismert pedigre adatokkal jol osszhangban all. | Cytokinin content (its type and concentration) was optimalized in the pre-treatment media in each cultivar examined (Royal Gala, M.26, Idared, Freedom, McIntosh, Husveti rozmaring) and the effects of different cytokinins on the morphogenic capacity were determined. Anatomical differences were detected in the leaves of in vitro plantlets treated with different cytokinins. There was a positive correlation between the histological status and regeneration capacity of in vitro leaves. Cytokinin content of regeneration media also was optimalized. Differences between effects of the different cytokinins and differences between the examined cultivars were proved considering the time curve of shoot regeneration. Molecular analysis of 65 apple cultivars were fulfilled by microsatellite primers (SSR) and we were able to distinguish the cultivars. Based on 6 SSR loci we prepared the dendogram of cultivars, which fits in with the well-known pedigree-data.