Chromatography of sugars on deae-spheron☆

Abstract Chromatography of borate complexes of sugars was carried out on diethylaminoethyl derivatives of glycol methacrylate macroporous gel, Spheron TM in borate form. The dependence of the separation of sugars on the exchanging capacity of the ion exchanger was tested. Sugar mixtures were best separated on an ion exchanger of maximum capacity 2.2 mequiv./g. The effect of the molarity of boric acid as well as the effects of temperature, flow-rate thourght the column, and boric acid gradient on the quality of the separation were investigated, and expressed by resolution and efficiency values (number of theoretical plates) and also by elution volume. For chromatography of the investigated mixture of sugars the following conditions were selected: buffer concentration 0.1 M , pH 8.5, temperature 50°, eluent flow, through a 500 x 6 mm I.D. column, 50 ml/h. The separation of a mixture of sugars on DEAE-Spheron under these conditions was compared with the separation on Aminex A-15. The separation efficiency for individual sugars was also compared on the basis of the number of theoretical plates achieved, and the values of un-reduced heights of the theoretical plates.