СЕЗОННЫЕ ВЛИЯНИЯ ПЕПТИДА GLY-PRO НА СЕКРЕТОРНУЮ ФУНКЦИЮ ЖЕЛУДКА У КРЫС С РАЗНОЙ РЕАКТИВНОСТЬЮ ЦЕНТРАЛЬНОЙ НЕРВНОЙ СИСТЕМЫ

The aim of this study was to identify the effect of seasonal changes of carbachol and Gly-Pro on secretor function of the stomach in rats with actively-search behavior, intermediate and passive-defensive behavior in the “open field”. The experiment was carried out on male rats of Wistar, the reactivity of the nervous system was assessed during the winter and summer solstice. Rats were imposed ligature on the pyloric section of the stomach, after that carbachol, Gly-Pro and saline were injected in various combinations. In 45 minutes of secretion, rats were decapitateв, gastric juice was collected, and its volume, acidity and proteolytic activity were identified. Statistical procedures were performed using STATISTICA 8.0, used the criterion of Mann-Whitney, cluster analysis. When comparing rates of gastric secretion after administration of carbachol, assessed in summer and winter periods, certain specific secretion features were detected only in rats with the passive-defensive type. This group demonstrated the smaller volume and greater proteolytic activity of gastric juice in summer compared to winter. Greater variety of secretion features was identified in thegroups of rats receiving the peptide cholinomimetic depending on the reactivity of the nervous system and season. In the summer the introduction of сholinomimetiс and Gly-Pro were accompanied with decreasing proteolytic activity of gastric juice in the rats with active search behavior; decrease in the volume of secretion, and in rats with an intermediate type of behavior; increase in volume of juice and decreased digestive ability in rats with passive-defensive type of behavior. In winter Gly-Pro on the background of carbachol increased only the acidity of gastric juice in rats with active search behavior in the “indoor field”, without affecting the levels of gastric secretion of two other groups of animals. Thus specific response of the secretory cells of the rats’ stomach after administration of carbachol solely, and together with Gly-Pro are determined by the reactivity of the central nervous system of animals and season of the year.