Fine Wool Sheep Superovulation and in vitro Lamb Embryos Production Transplantation

【Objective】The experiment was conducted to study the in vitro fertilization effect of lamb age,lamb weight,superovulation method sperm-oocyte co-incubation time on fine wool lamb superovulation and oocyte.【Method】 Domestic 4-9 weeks FSH lambs were chosen to go through two superovulation methods processing.1 032 oocytes available were recovered,and after in vitro fertilization and transplantation receptors were 98,lambing 25.【Result】The number of retrieved oocytes and oocyte number available of different weeks old lamb are greatly different(P0.05);the number of different hormone treatment group growth follicle two treatment options in the development showed no significant difference(P0.05),but the recovery rate and the number of oocytes available were significantly different(P0.05);there was a big difference(P0.05) between the growth follicle number and oocyte number available.【Conclusion】The results showed that 4 to 6 weeks of age of fine-wool sheep lamb superovulated oocytes obtained quite a lot of numbers,the number of repeated superovulation and the sheep superovulation effect is not ideal;at the same time,the semen pH value affected was adjusted to be between 7.5 and 7.6,and the sperm-oocyte co-incubation time control was within 22-26 h.Oocytes in vitro fertilization and development effect was good.