Direct measurement of in vitro antibody production using an enzyme-linked immunosorbent assay

Abstract A rapid and sensitive enzyme-linked immunoassay is described for quantitation of the secretion of cellular products during short-term (1–20 h) culture in vitro. The method is based on culture of cells, serially diluted in antigen or antibody-coated polystyrene microtire wells. The qwide applicability of this method for measurement of anti-hapten antibody, immunoglobulin and lymphokine production is stressed.