Developing bcl-x_(1) transgenic mice by microinjection

It was attempted to develop bcl-x_(1) transgenic mouse and produce its lineages for studying ischemic cerebrovascular diseases,bcl-x_(1) gene was introduced into mice by microinjection .The presence of bcl-x_(1) and its expression were confirmed by PCR,Southern blot,mRNA inspection,Western blot in the founders.In our experiments, bcl-x_(1) gene was injected into pronucleus of 1 654 zygotes and 1 448 injected eggs were implanted into 49 foster mothers, 7 of which were pregnant and bore 13 offsprings. Among the babies, 4 offsprings were integrated with the bcl-x_(1) gene and expressed it. Totally, implanted zygotes survival rate (83%), zygotes total survival rate (0.78%), recipient mouse pregnance rate (14.3%), integration rate (30.7%), expression rate(100%) ,total efficiency(0.24%)were obtained respectively. Bcl-x_(1) transgenic mice were obtained, 4 in number, but the transgenic efficiency was very low and the expression was unstable.