Hybrid modality photoacoustic/fluorescence microscopy through a multimodefiber using speckle illumination (Conference Presentation)
2019
We present an ultra-thin endoscope that combines a multimode optical fiber (MMF) attached to
an optical hydrophone for simultaneous optical-resolution photoacoustic microscopy and fluorescence
imaging. The MMF is used for light delivery and fluorescence collection and the hydrophone
for acoustic detection; a digital micro-mirror device (DMD) modulates the amplitude of the optical
wavefront of a pulsed laser coupled into the MMF, controlling the illumination at the distal tip.
The DMD allows for fast calibration approaches to reach calibration and measurement times of a
few seconds.
We obtain optical-diffraction-limited images with full field illumination recording the intensity
of a series of various calibrated speckle patterns produced by different configurations of the DMD
at the input, with no wavefront shaping. The intensity fluctuations from speckle pattern to speckle
pattern encodes for the position at which the signal is emitted. The fluorescence signal from the sample is
collected with the MMF and detected with a PMT at the proximal side. For the acoustic detection,
embedding the ultrasound detection within the device avoids the absorption of high-frequency ultrasound
by the tissue and therefore removes any limitation on the insertion depth. The footprint of
the probe is 250 um x 125 um making it thinner than common GRIN lenses used for endoscopy.
To best of our knowledge, our approach provides the thinnest endoscope head capable
of obtaining optical-resolution photoacoustic and fluorescence images simultaneously.
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