利用局部淋巴結分析(LLNA)偵測納乃得、谷速松及混合農藥之皮膚過敏性

Several types of pesticides, such as organophosphates and carbamate have a high risk of affecting human health, causing allergic rhinitis or allergic contact dermatitis. In recent years, allergic contact dermatitis has become an important subject in human life. In order to detect the potential chemicals allergens, the murine local lymph node assay (LLNA) has been developed and provided quantitative data suitable for dose response assessment. In this study, we designed to detect the potential skin sensitization of methomyl, azinphos-methyl technical pesticides, and 4 mixed pesticides by LLNA. The 2,4-dinitrochlorobenzene (DNCB) was used as positive control, and the 10% dibutyl phthalate (DBP) was added to increase the lymph node cells proliferative responses. The result of LLNA tests demonstrated methomyl, azinphos-methyl and other 3 mixed pesticides (include chlorpyrifos+cypermethrim, imidacloprid+carbofuran and imidacloprid+deltamethrin) with a low or negative skin sensitizing activity. DNCB and a mixed pesticide (carbendazim + hexaconazole) had a stimulated index (SI) more than 3. However, there was only a slightly increase or even reduction of the response after added 10% DBP. For the single pesticide of carbendazim and hexaconazole, the LLNA results showed that SI value were less than 3. In conclusion, methomyl and azinphos-methyl do not induce dermal sensitizing effects. In addition, not all of the skin sensitization of tested pesticides were augmented by DBP which may be regarded as partial enhances. For the mixed pesticides, we consider that the mixture of two pesticides may able to affect the skin sensitization. In order to avoid the potential hazard of pesticide allergens, appropriate protective measures should be taken for farmer and pesticide applicator, and we suggest the mixed pesticide products must provide the detailed skin sensitization information when registered.