Simultaneous determination of 9 compounds of saponins in PNS injections by RP-HPLC

Aim To establish a HPLC method for simultaneous determination of multiple components of saponins in PNS(panax notoginseng saponins)injections.Methods Zorbax eclipse XDB-C_(18)column(250 mm×4.6 mm,5 μm) and gradient elution was used.Solvent A was acetonitrile and solvent B was water.The gradient program of solvent A was as follows: 0~30 min,18%→19%;30~35 min,19%→35%;35~60 min,35% →55%.The flow rate was 1.5 ml·min~(-1).Diode array detector was set at 203 nm and column temperature was 40℃.Results The linear ranges of notoginsensode R_(1),ginsensode Rg_(1),Re,Rf,Rb_(1),Rc,Rb_(2)+Rb_(3)and Rd were 0.24~4.75 μg(r=0.999 9),0.57~11.40 μg(r=1.000 0),0.50~9.90 μg(r=1.000 0),0.24~4.80 μg(r=1.000 0),0.54~10.65 μg(r=1.000 0),0.26~5.10 μg(r=1.000 0),0.25~4.90 μg(r=1.000 0) and 0.32~6.30 μg(r=1.000 0) respectively.The recoveries of 9 compounds of saponins were between 99.3% and 100.2%.Conclusion 9 components of saponins in PNS injections could be simultaneously determinated by using the RP-HPLC method,which will improve the quality control.