Determination of cholecalciferol and related substances by calcium phosphate hydroxyapatite and calcium phosphate fluoroapatite high-performance liquid chromatography

Abstract A qualitative and comparative study by HPLC using two new stationary phases, hydroxy- and fluoroapatites, showed the separation under optimized conditions of vitamin D 3 and its related compounds, produced by irradiation and heating of provitamin D 3 .