Lipidomic Analysis of Glioblastoma Multiforme Using Mass Spectrometry

Glioblastoma multiforme (GBM) is the most common and malignant form of primary brain tumors. It is highly invasive and current treatment options have not improved the survival rate over the past twenty years. Novel approaches and technologies from systems biology have the potential to identify biomarkers that could serve as new therapeutic targets for GBM. This study employed Address correspondence to these authors at the Department of Technology Leadership and Innovation, Department of Agricultural and Biological Engineering, Bindley Bioscience Center, Purdue University, 155 S. Grant Street, Young Hall 367, West Lafayette, IN 47907, USA; Tel: (765) 494-4649; kclase@purdue.edu;. Herman B Wells Center for Pediatric Research, Riley Hospital for Children at IU Health, 1044 West Walnut Street R4 302, Indianapolis, IN 46202-5525, USA; Tel: (317) 274-8891; kpollok@iu.edu. AUTHORS’ CONTRIBUTIONS Kari Clase was responsible for leading the collaboration, analyzing data and interpreting findings within the context of the biological system and establishing the data collection and sample analysis pipeline. Soo Jung Ha and Gordon M. Showalter conducted the sample preparation, mass spectrometry data collection and statistical data analysis. Jiri Adamec supervised mass spectrometry data collection. John Springer conducted statistical clustering analysis of mass spectrometry data. Shanbao Cai, Haiyan Wang, Wei M. Liu were directly responsible for intellectual input on model set up. They also set up all flank and orthotopic model, mouse monitoring, and isolation of tumor samples. Karen Pollok was responsible regulatory requirements, intellectual input, and all aspects of in vivo model set up, monitoring, and sample collection. Jenna Rickus provided intellectual input. Jann Sarkaria provided tumor cell lines and intellectual input. Aaron Cohen-Gadol provided intellectual input. CONFLICT OF INTEREST The authors confirm that there is no conflict of interest with the data presented in this article. HHS Public Access Author manuscript Anal Chem. Author manuscript; available in PMC 2016 April 29. Published in final edited form as: Anal Chem. 2007 November 15; 79(22): 8423–8430. doi:10.1021/ac071413m. A uhor M anscript