Background Reduction in STED-FCS Using a Bivortex Phase Mask

Aurélien Barbotin,Iztok Urbančič,Silvia Galiani,Christian Eggeling,Martin J. Booth

Background Reduction in STED-FCS Using a Bivortex Phase Mask

2020

Aurélien Barbotin
Iztok Urbančič
Silvia Galiani
Christian Eggeling
Martin J. Booth

Fluorescence correlation spectroscopy (FCS) is a valuable tool to study the molecular dynamics in living cells. When used together with a super-resolution stimulated emission depletion (STED) micro...

Keywords:

Fluorescence correlation spectroscopy
STED microscopy
Background noise
Stimulated emission
phase mask
signal quality
Optics
Physics
Optoelectronics

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