Respiration shutoff in Escherichia coli K12 strains is induced by far ultraviolet radiations and by mitomycin C

Abstract Ultraviolet radiations (254 nm) (UV) cause respiration to shutoff in Escherichia coli B/r. It has been reported [P.A. Swenson, Photochem. Photobiol., 33 (1981) 855–859 and J. Barbe, A. Vericat and R. Guerero, Mutation Res., 120 (1983) 1–5] that E. coli K12 strains do not shut off respiration after UV. The latter authors also reported that mitomycin C did not cause this ‘SOS’ response. In this paper we report that higher UV fluences than were previously used will cause respiration shutoff in K12 strain W3110 and that cyclic AMP increases the sensitivity of respiration shutoff of irradiated cell suspensions. We also report that mitomycin C shuts off respiration in this strain. Neither UV nor mitomycin C causes respiration shutoff in the rec A56 derivative of W3110. Thus respiration shutoff is a recA dependent response to UV and mitomycin C in E. coli K12 strains.