Effect of temperature on guinea pig urinary bladder contraction mediated via P2X-receptors.

In vitro experiments showed that P2X-receptor agonist α,β-methylene-ATP and electrical field stimulation in the presence of muscarinic and α-adrenoreceptors blockers induced contractile responses of isolated guinea pig bladder, which were more pronounced at 30°C than at 37°C or 42°C. P2x-receptor antagonist pyridoxal-6-phosphate-2′,4′-disulfonic acid, produced a more potent inhibitory effect on contractions induced by electrical field stimulation at 30°C in comparison with that at 37°C or 42°C while the contractions induced by α,β-methylene-ATP were similarly suppressed at all examined temperatures.