Alloimmune Response Results in Expansion of Autoreactive Donor CD4+ T Cells in Transplants That Can Mediate Chronic Graft-versus-Host Disease
2011
Chronic graft-versus-host disease (cGVHD) is considered an autoimmune-like disease mediated by donor CD4 + T cells, but the origin of the autoreactive T cells is still controversial. In this article, we report that the transplantation of DBA/2 donor spleen cells into thymectomized MHC-matched allogeneic BALB/c recipients induced autoimmune-like cGVHD, although not in control syngeneic DBA/2 recipients. The donor-type CD4 + T cells from the former but not the latter recipients induced autoimmune-like manifestations in secondary allogeneic BALB/c as well as syngeneic DBA/2 recipients. Transfer of donor-type CD4 + T cells from secondary DBA/2 recipients with disease into syngeneic donor-type or allogeneic host-type tertiary recipients propagated autoimmune-like manifestations in both. Furthermore, TCR spectratyping revealed that the clonal expansion of the autoreactive CD4 + T cells in cGVHD recipients was initiated by an alloimmune response. Finally, hybridoma CD4 + T clones derived from DBA/2 recipients with disease proliferated similarly in response to stimulation by syngeneic donor-type or allogeneic host-type dendritic cells. These results demonstrate that the autoimmune-like manifestations in cGVHD can be mediated by a population of donor CD4 + T cells in transplants that simultaneously recognize Ags presented by both donor and host APCs.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
62
References
34
Citations
NaN
KQI