Smad3 Modulates Tendon Gene Expression and Interacts with Key Tendon Transcription Factors

METHODS Gene Expression qPCR Analyses: Hindlimb and tail tendons were isolated from 6-week old mice. RNA was extracted using the Purelink RNA Mini Kit (Invitrogen) and cDNA was reverse transcribed using the iScript cDNA synthesis kit (BioRad). Expression was assessed by SYBR green fluorescence during qRT-PCR with established primers. Data was analyzed using a two-tailed t-test assuming unequal variances.