Phenotype analysis and mutant gene location of ventral yellow mouse (VY(Slac))
2013
The ventri-yellow pigmentation mouse (temporarily named VYSlac) arose
spontaneously in the C57BL/6J inbred mouse strain, found and bred by
Shanghai SLAC Laboratory Animal Co., Ltd. VYSlac presented a special
phenotype marked by yellow coat on the ventral surface of neck and
trunk that was without melanin deposition but maintained a normal
structure. The number of melanocytes in epidermis and melanin in hair
follicle of the abdominal skin of the mutant mouse were less than that
of their background strain, while there was no significant difference
between the dorsal skins of the two strains. This mutant phenotype was
inherited as single-gene dominant inheritance, confirmed by genetic
experiment, and there was no significant difference between VYSlac and
B6 for other biological parameters such as weight, anatomic and
histological structures of major organs and blood physiology. When the
linkage relationship between the genomic DNA samples of F2 48 mice
(VYSlacD2F1×D2) and mutant phenotype were evaluated, the mutant
gene was confirmed on chromosome 2 near D2Mit229. New microsatellite
and SNP markers were selected to amplify genomic DNA samples of 196 F2
mice and the mutant gene was narrowed down to 5.3 Mb region between
rs13476833 and rs27310903 on chromosome 2. The preliminary results of
our phenotype analysis and gene location provides a solid basis for
further identification of this mutant gene.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
14
References
0
Citations
NaN
KQI