A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro

Yeast and other eukaryotic cells contain distinct compartments that have specific roles. For example, compartments called mitochondria – which are surrounded by two layers of membrane – provide the energy needed for many cell processes. The organization of the network of mitochondria in a cell has a large effect on their capacity to provide energy. Mitochondria can fuse together to make larger compartments or divide to make smaller ones. Defects in fusion or division of mitochondria can reduce the amount of energy that is provided, which, in humans and animals can lead to diseases that affect various organs, especially those in the nervous system. When two mitochondria fuse they must first attach to each other and then merge their outer membranes. Proteins called mitofusins are known to be involved in these processes, but the molecular details of how they take place were not clear. Brandt, Cavellini et al. investigated how mitochondria isolated from budding yeast cells attach to each other. The experiments found that two mitochondria first become loosely attached by mitofusins. These proteins then promote a tighter attachment in which the outer membranes of the two mitochondria come into contact over a larger area. This contact area is determined by a linear arrangement of proteins referred to as the docking ring. Brandt, Cavellini et al. further observed that local fusion between the outer membranes takes place at the edge of the contact area in the path of the docking ring. Future research will need to address how mitochondria attach to each other in living cells and how the process is regulated.