In Vitro Evaluation of the Efficacy of Sodium Humate as an Aflatoxin B1 Adsorbent

In vitro adsorption of aflatoxin B1 (AFB1) on sodium humate was investigated by using Freundlich and Langmuir isotherms and the Freundlich isotherm fitted the data better than the Langmuir isotherm. At the same time, effects of PH, interaction time, adsorbent amount and initial concentration of AFB1 were also studied and they all had a certain influence on AFB1 adsorption, especially the PH value. The coexistence of fresh feed(AFB1<1ng/g)showed no significant influence on AFB1 adsorption, indicating that sodium huamate have a high affinity to AFB1 and don't adsorb other nutrients. The AFB1-adsorbent complex was stable in phosphate buffer solution at PH 3.0 and PH 8.0. These results suggest that sodium humate have the potential as an aflatoxinB1 adsorbent to prevent the adsorption of AFB1 from the animal gastrointestinal tract.