Simple electrochemical detection of Listeria monocytogenes based on a surface-imprinted polymer-modified electrode.

Listeria monocytogenes (LM) is a foodborne pathogen and it can pose a serious of diseases to the human health, hence developing effective method for detecting LM is very important. In this work, by selecting LM as template and 3-thiopheneacetic acid as functional monomer, a LM-imprinted polymers (LIP)-based sensor was proposed for the first time to detect LM by electro-polymerizing TPA on the glassy carbon electrode (GCE) surface in the presence of LM. After removing LM template from the electrode surface, the obtained sensor was denoted as LIP/GCE which can effectively recognize and capture LM cells. By using [Fe(CN)6]4-/3- as probe, its peak current at LIP/GCE can be restricted when LM cells were captured to the imprinted cavity of LIP/GCE, and the current value decrease with the increase of LM concentration. A serious conditions were optimized for achieving highly sensitive detection, and a low detection limit (4 CFU mL-1) coupled with wide linear range (10 - 106 CFU mL-1) were obtained for LM. Finally, the repeatability, stability and selectivity as well as applicability of LIP/GCE were also investigated, and the exhibited results were acceptable.