Abstract 47: Point-of-Care Seeded Blood-Derived Endothelial Cells Rapidly Endothelialize Nitinol Stent in a Pig Model of Personalized Cell Therapy

Intimal hyperplasia after percutaneous intervention is especially problematic in peripheral arteries. In coronary arteries, drug-eluting stents (DES) have greatly reduced intimal hyperplasia; however, DES not only prevent smooth muscle cell proliferation but also inhibit endothelial cell (EC) healing. In an effort to rapidly endothelialize Nitinol vascular stents, we have developed a novel rapid seeding technology (QuickSeeding). 5000 micropores (~32.4 ± 0.4 μm) were laser-drilled circumferentially into Nitinol stent delivery systems to enable ECs to flow across the stent struts and thereby attach to the stent while in its compressed state within 5 min at the point-of-care. To evaluate EC adhesion after stent deployment, ECs were isolated from human umbilical cord blood progenitor cells and QuickSeeded, resulting in a uniform stent surface coverage of 55,000 ± 9,500 cells/cm2 (n=4). QuickSeeded stents were then deployed in tubing and tested in a flow circuit at 15 dynes/cm2, representing arterial flow she...