Comparison of various diagnostic kits and antigens for the detection of chlamydial antibodies in the serum of patients with respiratory diseases

: Examination of sera of patients with respiratory diseases did not reveal any substantial difference in detection of chlamydial antibodies by ELISA using two commercial kits (Chlamydia STAT and rRLISA) demonstrating IgG antibodies and two corpuscular antigens prepared from Chlamydia psittaci and Chlamydia pneumoniae in the Institute of Virology, SAS, in Bratislava detecting whole serum antibodies. However, higher sensitivity of rELISA diagnostic kit was found when analyzing immunoglobulin classes, namely in case of IgG antibodies. Results of ELISA were in a great majority of cases, confirmed by examination of sera by microimmunofluorescence test, corpuscular antigens from the Institute of Virology being somewhat more sensitive than the commercial Micro-IF test kit. (Tab. 4, Ref. 23.)