Method for room temperature amplification of DNA

The invention discloses a method for room temperature amplification of DNA. The method comprises the following steps: (1) extracting template DNA; and (2) mixing the template DNA with an amplification reagent to form an amplification mixed liquor and then carrying out room temperature DNA amplification for 10 to 60 min so as to obtain desired amplified DNA. The method provided by the invention realizes amplification of DNA at normal temperature, makes DNA amplification possible under the condition of limited resources, shortens reaction time, prepares the specific amplification product and simplifies pairing of DNA primers and extension of a DNA polymerase compound.