Hidrogéis a base da associação de Rhizophora mangle e ácido ascórbico : desenvolvimento e avaliações citotóxica e imunomoduladora

Galenic development of topical herbal medicines has been the subject of frequent research, including products with healing action. Among the great arsenal of the Brazilian flora, Rhizophora mangle (R. mangle). Ascorbic acid is a fundamental active in the synthesis of collagen and elastin, acting in tissue repair being in this work associated with the freeze-dried aqueous extract of R. mangle with the purpose of stimulating the healing action. Object of this study, presents, among its phytoconstituents, the polyphenols, substances with known healing and anti-inflammatory action. Given the above, this study aimed to develop and evaluate cytotoxic and immunomodulatory flow cytometry of R. mangle + Ascorbic Acid hydrogels. In the preformulation study three different gelling polymers, the polymers chosen as the base for the hydrogel were tested followed by evaluation of organoleptic characteristics, pH determination, rheology and spreadability. In the preclinical cytotoxic evaluation study mouse splenocytes (10⁶ cells / well) were treated with formulations at concentrations of 0,05%, 0,1% and 0,2% for cell viability analysis using annexin V and propidium iodide. Then at the concentration of 0,1% the cell proliferation assay was performed with 5- (6) -Carboxyfluorescein-diacetate-N-succinimidyl ester (CFSE), the measurement of cytokine levels in lymphocyte supernatants by the Cytometric Beads Matrix System. (CBA) and evaluation of lymphocyte activation and inhibition also for the immune profile. The polymer selected as the basis of the formulations was 1,5% xanthan gum, which were homogeneous, orange in color, smooth surface, intense gloss, typical odor of the actives, non-Newtonian pseudoplastic behavior and pH between 5,0 and 5,3. The result of the preclinical evaluation demonstrated the non-cytotoxic profile, inducing proliferation, activation of CD4 ⁺ T, CD8 ⁺ T and NK T lymphocyte subunits, as well as the production of IL-2, IL-4, IL-10, IL- 17 and TNF-α in stimulated splenocytes. The results suggest that R. mangle + Ascorbic Acid hydrogels may be promising preparations in the wound healing process.