The Effects of Polyene Phosphatidylcholine on TNF-α mRNA Level in NAFLD Rats

Objective:To detect and analyze the effects of medical intervention for non-alcoholic fatty liver disease(NAFLD) using reverse transcription(RT) and real-time quantitative polymerase chain reaction(real-time qPCR).Methods: The Wistar rats were fed with high fatty diet for 12 weeks to induce NAFLD,and then were switched to low fatty diet,intervened with polyene phosphatidylcholine,compared with controls receiving distill water for 4 weeks.Each group consisted of 8 rats.The total RNA in adipose tissue was extracted and reversely translated into cDNA for detection of the relative level of tumor necrosis factor α (TNF-α) using real-time qPCR.T-test was performed for statistical significance of the data obtained.Results: The relative level of TNF-α mRNA in adipose tissue of intervention group(0.269±0.047) were higher than that of control group(0.125±0.015).The difference of TNF-α mRNA between the two groups was significant(t=3.094;P=0.009).Conclusion: Polyene phosphatidylcholine can significantly increase the expression of TNF-α gene in adipose tissue of NAFLD rats undermining glucose metabolism in liver.