Total Phenolic Content, Reducing Potential & Free Radical Scavenging Activity of HydroalcoholicLeaf Extracts of Psidium guajava and Persea americana

In the present study, we have investigated the total phenolic content reducing property and antioxidant activity of hydroalcoholic leaf extract of Psidium guajava and Persea americana. Psidium guajava belongs to the family Myrtaceae, and is distributed throughout India. Earlier studies involving the leaves, roots and fruits of this plant, displayed ethno medicinal properties, and was used in treatment of various diseases. Tender leaves of Psidium guajava displayed antioxidant, antibacterial, anti-inflammatory, astringent and hypoglycemic activity. Persea americana belongs to family Lauraceae and is found abundantly in tropical and temperate regions of the world. The leaf extract significantly reduced the blood glucose level and helped in the management of diabetes. In view of this, hydro alcoholic leaf extracts of Psidium guajava and Persea americana were studied for their antioxidant properties like ABTS free radical scavenging activity and reducing property .Their total phenolic content was also estimated. Experimental results clearly unveil the antioxidant property of both plant extract. At 10 μg/ml concentration Psidium guajava and Persea americana leaf extract exhibited 28.48 ± 2.06 & 24.67 ± 3.4 % ABTS free radical activity,whereas standard ascorbic acid displayed 77.67 ± 0.28 respectively .The maximum phenolic content was found in Psidium guajava leaf extract ( 32.5 ± 4.33 mg GAE/g of extract) & minimum in (28.33 ± 2.88 mg GAE/g of extract) Persea americana. In the present study we observed that Psidium guajava leaf extract has maximum free radical scavenging, reducing potential and phenolic content when compared with Persea americana. Results indicated an increase in free radical scavenging activity and reducing property in both the plants in a dose dependent manner which was directly related to the phenolic content present in their leaf extracts.