Cell culture and characterization of solid adamantinomatous craniopharyngioma

Objective To establish an efficient method of primary solid adamantinomatous craniopharyngioma cells culture and observe the behaviour of cells in vitro.Methods The specimen of solid adamantinomatous craniopharyngioma were treated with trypsin and cultured in vitro,and purified by filter membrane.The cell morphology was observed by inverted microscope and hematoxylin eosin staining.The expression of Pan -ck was examined by immunocytochemistry.The MTT assay and flow cytometry were employed to study the cell growth and proliferation.Results The cell morphology was similar to that of typical epithelial cell and the staining of Pan - ck was positive in immunocytochemistry.The cultured tumor cells grew slowly and were all diploid.Conclulions The adamantinomatous craniopharyngioma cells could be obtained by enzymatic digestion method and special culture media.Filter membrane could be applied to improve the purity and success rate of craniopharyngioma cells. Key words: Craniopharyngioma,adamantinous; Cell culture technique