Effect of Buqi huoxue Decoction on Apoptosis in Chronic Bronchitis Rats with Deficiency of Lung-qi

Objective To explore the effect of Buqi huoxue decoction on apoptosis of chronic bronchitis rats with deficiency of lung-qi. Methods Ten rats were randomly selected from 50 SD rats as control group,and models of the deficiency of lung-qi of chronic bronchitis( CB) were established using the improved smoking-fumigated method in the other40 rats. The successful model rats were randomly divided into model group,high-dose group of Buqi huoxue decoction,low-dose group of Buqi huoxue decoction and tonifying lung recipe group( n = 10 for each group) by random digits table.The control and model groups underwent normal saline irrigation,while the other three groups were treated with high and low doses of Buqi huoxue decoction and tonifying lung recipe. Apoptosis conditions were observed with TUNEL kit and flow cytometry. Results The percentage of inflammatory cells apoptosis in model group was significantly lower compared with that in control group( P 0. 05),and the percentage of lung parenchyma cell apoptosis was significantly higher compared with that in control group( P 0. 05); the percentages of inflammatory cells apoptosis in high-dose,low-dose and tonifying lung recipe groups were significantly higher compared with that in model group,and the percentage in high-dose group was significantly higher compared with those in low dose and tonifying lung recipe groups( P 0. 01); the percentages of lung parenchyma cell apoptosis in high-dose,low-dose and tonifying lung recipe groups were significantly lower compared with that in model group,and the percentages in high-dose and low-dose groups were significantly lower compared with that in tonifying lung recipe group( P 0. 01,P 0. 05). Conclusion The Buqi huoxue decoction can improve deficiency of lung qi of pulmonary tissue morphology in chronic bronchitis rats. The mechanisms of controlling inflammation and strengthening the lung tissue self-repair may be related to the apoptosis induction of inflammatory cells in lung tissue and inhibition of lung parenchyma cell apoptosis.