DNA fragment with E2F1 protein binding property and application to E2F1 activity detection

The present invention relates to a kind of combinative DNA fragmentation of E2F1 albumen, it includes one or more E2F1 protein binding frames；Further relate to the application of the DNA fragmentation；Further relate to a kind of method for detecting intracellular E2F1 transcriptional regulatory activities.By using DNA fragmentation of the invention and method, the transcriptional regulatory activity of intracellular E2F1 can be detected directly against property, rather than the content of its transcript or protein is only detected, it is enable to more accurately analyze effects of the E2F1 as transcription factor played in some Developmental Biology and pathological development.