Cloning and Differential Expression of Acidiphilium Cryptum DX1-1 CO_2 Fixed-Related Gene

Objective: To investigate the energy substrates impact on the expression of genes related with CO2 fixed in Acidiphilium cryptum DX1-1.Methods: DNA of Acidiphilium DX1-1(CCTCC M 208056) was used as template.The annotation of A.cryptum DX1-1 genes and their putative cellular functions were from Comprehensive Microbial Resource(CMR) of A.cryptum JF-5(JGI,http://genome.ornl.gov/cgi-bin/JGI_microbial /kegg_categories.cgi).Results: The genes related with CO2 fixed-Acry_0824,Acry_082,Acry_1067,Acry_1272,Acry_0022 and Acry_0827 were cloned successfully.The RT-qPCR result shows that in mixotrophy with addition of limited 0.1% glucose,the expression of Arcy_0824,Arcy_1272,Arcy_0827,were significantly upregulated in the mixotrophy comparing to that in heterotrophy and autotrophy.Conclusion: PHB metabolism of DX1-1 is more activated in mixtrophy and utilizing CO2 to accumulate PHB.